Research Outputs
Inter-sexual comparison of body biomass, proximate biochemical composition, and fatty acid profiles of new juvenile squat lobsters (Pleuroncodes monodon) in the Southeast Pacific Ocean
In the wide distribution range of the red squat lobster Pleuroncodes monodon in the Southeast Pacific Ocean, there is an important nursery area on the southern coast of Chile. The new juvenile individuals from this nursery area are directly recruited into the adult squat lobster population, which is exploited by industrial fisheries. Despite the importance of new P. monodon juveniles for recruitment estimations in fishery management models, their bioenergetic condition and/or nutritional status at the onset of their benthic phase remain unknown, as are the differences in the biochemical composition and energy reserves of the two sexes, which could help explain the cost of the first breeding event in females. Therefore, in new juvenile squat lobsters with the same degree of immaturity, we quantified and compared between the sexes (female vs. male): the size (cephalothorax length, CL), body biomass (dry weight and organic matter), biochemical composition (proteins, glucose, and lipids), and fatty acid profiles (FAs). The results indicate that the CL of new juveniles was similar between the sexes, while the dry weight and organic matter presented significant differences, with higher values in new juvenile females (NJF) than new juvenile males (NJM). Similarly, the NJF had a higher content of proteins, glucose, and lipids than NJM. The FAs also showed significant differences between the sexes; the NJF had a higher content than NJM in all fatty acid classes (i.e., saturated, monounsaturated, and polyunsaturated), with the FAs DHA (C22:6n‐3), EPA (C20:5n‐3), oleic (C18:1n‐9), palmitic (C16:0), and eicosatrienoic (C20:3n‐3) significantly contributing to the differentiation of FA profiles between the sexes. All of the aforementioned differences among the multiple variables of the bioenergetic condition can indicate biochemical adaptations in the storage capacity of energy reserves, particularly among NJF that must support the high energy cost of the first reproductive event (characterized by embryo production and incubation), which occurs during the austral winter, a period predominated by cold‐water temperatures and scarce food availability in the habitat. Overall, our findings have significant implications in fishery management models. In this case, defining the bioenergetic condition of the new juvenile squat lobsters can aid in predicting the density and stability of the adult population, which is exploited by commercial fisheries.
Contrasting nursery habitats promote variations in the bioenergetic condition of juvenile female red squat lobsters (Pleuroncodes monodon) of the Southern Pacific Ocean
The red squat lobster Pleuroncodes monodon is an important fishery resource in the Humboldt Current System (HCS). This decapod is exploited in two fishing units: (a) the northern fishing unit (NFU, from 26°S to 30°S) and (b) the southern fishing unit (SFU, from 32°S to 37°S), each of which have an adjacent nursery area that is the source of recruits to replace the exploited adult populations (in the NFU: off the coast of Coquimbo (28°S) and in the SFU: off the coast of Concepción (36°S)). Marked spatial differences in the environmental conditions of the NFU and SFU, and the biogeographic break that exists between these nursery areas (30°S) may promote changes in the bioenergetic condition of new P. monodon juveniles. To evaluate this, we analyzed the bioenergetic condition (measured as: body mass, lipids, proteins, glucose, and energy) of new juvenile females in the main nursery areas off the Chilean coast. The juvenile females from the SFU showed a higher body mass than those from the NFU. Consistently, the juvenile females from the SFU had a higher content of lipids, proteins, and glucose than those from the NFU, indicative of higher energy contents and a higher lipid/protein ratio in the south compared to the north. Considering the current overexploitation of this fishery resource in the HCS, it is essential to understand how the bioenergetic condition of juvenile females of P. monodon may vary in nursery areas at different latitudes in order to generate sustainable fishery management policies with an ecological approach, designed specifically to each fishing unit. Furthermore, identifying the latitudinal variations of these biochemical compounds in P. monodon juveniles can elucidate the geographic origin of red squat lobsters that present a ”better bioenergetic condition” in the HCS, which may significantly benefit sustainable fishing certification processes.
Spatio-temporal changes in the biochemical parameters of the fishery resource Concholepas concholepas (Gastropoda: Muricidae) in the Southeastern Pacific Ocean
The fishery resource Concholepas concholepas is a key species in the benthic marine ecosystems of the Southeastern Pacific Ocean. In the present study, the spatio-temporal changes in the biochemical parameters were evaluated for adult individuals of C. concholepas. They were sampled in summer and winter under upwelling and Non-upwelling conditions in two Management and Exploitation Areas for Benthic Resources of Valparaíso, Chile. The results indicated that the variation in the biochemical parameters is explained to a greater extent by the season. For example, in both upwelling and Nonupwelling conditions, we found a higher content of total lipids, proteins, glucose, and energy in winter than summer. Temporal variations can be explained by the reproductive process after summer season, in which the egg spawning require a greater energy reserves to be perform. Nonetheless, differences in the nutritional status of preys along seasons of the year, can also influence on these results. In fact, these variations in the biochemical parameters of C. concholepas may be indicative of an adaptive seasonal physiological response to the environment for maintaining an optimal energy budget year-round. Altogether, this knowledge will contribute to the Management and Exploitation Areas for Benthic Resources, improving regulatory measures during the annual period of captures and landings of C. concholepas, favoring a more sustainable fishery in the Southeastern Pacific Ocean within an ecosystem approach.
How does emersion time affect embryos of coastal marine invertebrate species? Biochemical responses of three porcelain crabs from the Southeastern Pacific
Porcelain crabs that inhabit contrasting environments along the Southeastern Pacific coast have developed physiological adaptive responses to the harsh environmental conditions. However, it is not yet known if these responses are already present in early life stages, in embryos, for example, which are more vulnerable to environmental fluctuations than adults. In this study, we subjected ovigerous female crabs of three crab species (Allopetrolisthes punctatus,Petrolisthes laevigatus, andPetrolisthes violaceus) to different periods of emersion (0, 1, 3, and 5 hr) to measure the weight and biochemical parameters (i.e., glucose and lactate) in their embryos after a period of stress induction through emersion. The results indicate that after five hours of emersion,P. laevigatus' embryos had the highest dry weight. This same trend was observed in the embryos ofP. violaceus.Allopetrolisthes punctatus' embryos had the lowest weight. The embryos' glucose content inP. laevigatusandP. violaceuswas consumed during the emersion time, while in embryos ofA. punctatusit remained relatively constant. The highest glucose content was reported inP. laevigatus, followed byP. violaceusandA. punctatus. The lactate content showed an opposite trend and was gradually accumulated with increasing emersion time.Petrolisthes laevigatushad the highest lactate content. This study reveals that the early ontogeny of these porcelain crabs is well adapted to long periods of emersion, showing biochemical adaptive responses linked to aerobic metabolism. These adaptations could reflect a distinctive physiological trait, explaining why porcelain crabs are able to survive in contrasting conditions.
Consumption of marine-derived nutrients from invasive Chinook salmon (Oncorhynchus tshawytscha) transfer ω-3 highly unsaturated fatty acids to invasive resident rainbow trout (O. mykiss)
Marine-derived nutrients (MDN) contained in gametes (mature eggs and sperm), carcasses and metabolic wastes from anadromous migratory salmon can transfer energy and materials to fresh water, thereby affecting the structure and function of stream ecosystems. This is crucial among ecosystems where humans have mediated biological invasions by propagating non-native species. Previous studies have demonstrated that consumption of MDN from salmon can benefit both native and invasive resident fishes. Yet, a more detailed understanding of the transfer of biomolecules with important physiological functions such as ω-3 highly unsaturated fatty acids (HUFAs) have received less attention among researchers. Here we demonstrate that consumption of MDN contained in invasive Chinook salmon eggs transfers ω-3 HUFAs (e.g., EPA and DHA) to resident invasive rainbow trout in a river food web. We conducted a field study in river sections previously identified as spawning areas for Chinook salmon in the Cisnes River, Patagonia. Rainbow trout were sampled around salmon spawning areas before, during, and after the salmon spawning season. Additionally, we collected tissue from different food web resources and components of different origin (e.g., primary producers, aquatic and terrestrial items) from the Cisnes River system. Analyses of stomach contents of trout were performed in conjunction with analyses of both lipid content and fatty acid profiles of trout tissue and food web components. Chinook salmon eggs showed higher content of ω-3 HUFAs, especially EPA (31.08 ± 23.08 mg g DW−1) and DHA (27.50 ± 14.11 mg g DW−1) than either freshwater or terrestrial components (0–6.10 mg g DW−1 both EPA and DHA). We detected marked shifts in the fatty acid profile (~six-fold increase in EPA and DHA) of trout following consumption of Chinook salmon eggs. Our findings suggest that MDN via consumption of salmon eggs by resident rainbow trout may positively influence resident trout and likely contribute to gauge synergistic interactions between invaders on receiving ecosystems of Patagonia.
Upwelling enhances seaweed nutrient quality, altering feeding behavior and growth rates in an intertidal sea urchin, Loxechinus albus
Upwelling systems deliver nutrient-rich water into coastal ecosystems, influencing primary productivity and potentially altering seaweed-herbivore interactions. Upwelling bottom-up effects on distinct trophic levels are wellknown. However, their influence on seaweed biomolecules and on algae-herbivore interactions and growth are less known. The aim of this study was threefold: i) to compare physical-chemical characteristics and nutrient levels in the water of upwelling (U) and downwelling (DU) zones, ii) to quantify their influence on the content of protein and carbohydrates in seaweed tissues of representative U and DU locations, and iii) to experimentally assess their effect on the feeding behavior and growth of a prominent intertidal herbivore, the sea urchin Loxechinus albus. Waters from U zones showed lower temperatures and pH, and higher phosphate concentrations than those from downwelling zones. Similarly, the tissue of seaweeds from a U location had significantly more proteins and carbohydrates than those from a DU location. The origin location of the sea urchins had a significant influence on consumption and growth rates: in general, those coming from a site with U conditions consumed and grew more than those coming from DU conditions. The quality of the algae was a significant factor on consumption rates, although in the case of preference trials, this factor interacted with sea urchin origin location. Our results show that the availability and quality of the food in upwelling zones has an influence on herbivore-seaweed direct interactions. However, these interactions and the growth of the sea urchins were also related to the coastal site and conditions from which the sea urchins came from. These results are relevant considering the expected impact of climate change on the world's oceans, and the importance of U zones as thermal (cold water) refuges for marine ectotherms.
Seasonal dynamics of biochemical composition and fatty acids of swordfish (Xiphias gladius) in the Southeast Pacific Ocean off the coast of Chile
In the Southeast Pacific Ocean, Xiphias gladius migrates through the Chilean coastal zone for feeding. Here, it forages for different prey items from autumn to spring, acquiring a great variety of energy and nutritional reserves. We evaluated seasonal variations in the biochemical reserves (i.e., contents of lipids, proteins, and glucose), total energy content and fatty acid profile of specimens captured during the austral autumn, winter, and spring. Our results show that higher amounts of lipids were found in the winter and spring, while protein and glucose were higher in the autumn. Thus, the energy content showed significant differences, with higher levels in winter and spring. Furthermore, the fatty acid profile was more diverse in the spring than the autumn and winter and was characterized by higher amounts of polyunsaturated fatty acids. These findings suggest that temporal changes in the biochemical reserves, total energy content and fatty acid profile support the idea of a “trophic migration” (i.e., the feeding period) established by the dynamics of fishery fleets. The high amounts of lipids and diverse fatty acid profile found in the spring could indicate the end of the trophic migration during this season. Thus, X. gladius may reach an optimum nutritional condition in the spring and make energetic adjustments to carry out its reproductive migration during the austral summer. Therefore, this species seems to meet the high energy demands of the reproductive season by foraging for a wide range of prey items from autumn to spring and storing an increased amount of lipids at the end of the feeding period. Overall, our data provides crucial baseline knowledge for future research on the ecophysiology of X. gladius, as well as for the management and conservation of this fishery resource under an ecosystem approach.
The paralytic shellfish toxin effect on bioenergetic constituents of the fishery resource Chorus giganteus (Gastropoda: Muricidae)
Alexandrium catenella, one of the most common harmful microalgae observed in southern Chile, produces paralytic shellfish toxins, which can affect many organisms throughout the trophic chain. This research evaluated how paralytic shellfish toxins affected the principal bioenergetic constituents and fatty acids composition of the carnivorous snail Chorus giganteus. Snails were separated into a “toxic” group that was fed the toxic clam Mulinia edulis (which was previously fed A. catenella), and a “non-toxic” group, fed non-toxic clams. Both groups were kept under these conditions for 63 days. Our results indicated no difference in the ingestion rate of toxic versus non-toxic snails; however, a higher protein level was identified in toxic snails. The total lipid content proved to be no different in toxic versus non-toxic snails; although, an effect of the toxic diet on the fatty acid profile of C. giganteus was observed. High levels of essential polyunsaturated fatty acids, especially docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) in toxic snails, were identified. Our results suggest that exposure to paralytic shellfish toxins, through diet, may cause changes in the biochemical composition of C. giganteus, which may have a subsequent impact on its energetic physiology.
Trophodynamics of the jumbo squid Dosidicus gigas during winter in the Southeast Pacific Ocean off the coast of Chile: Diet analyses and fatty acid profile
Marine trophodynamic studies have been developed mainly through the evaluation of stomach contents. However, these studies only reflect the feeding of a few days, showing a "snapshot" of the food consumed for a short time. Currently, new complementary techniques have been developed to evaluate the diet of predators using biochemical markers (fatty acids, FAs) and can, in turn, consider the rate of renewal of these biochemical constituents in body tissues. In the jumbo squid Dosidicus gigas, an important fishery resource in the Humboldt Current System, we identified and analyzed the stomach contents (prey) of D. gigas collected along the coasts of Chile in order to compare the FA profiles of its prey with those of three of its principal tissues and/or organs (digestive gland, gonad and mantle muscle). The analyses of the stomach contents indicate that D. gigas feeds on fish, crustaceans and squids, as previously have been reported. Regarding the FAs, the digestive gland presented the highest concentration and diversity of FAs, followed by the gonad and then the mantle. In turn, when comparing the FAs of the digestive gland of D. gigas with the FA profiles of its preys, the crustacean Pleuroncodes monodon and the fish Lampanyctus sp. presented the closest similarity, especially with a high contribution of essential FAs and PUFAs. Our findings indicate that during the cold season (austral winter), individuals of D. gigas may present an energy optimization strategy, characterized by the intake of prey with a high energy content rich in PUFAs, such as crustaceans and small fishes. This study not only contributes to the understanding of the squid biology and their trophodynamic, but also has important implications to improve fishery management within an ecosystem approach. This is relevant because D. gigas and its prey have suffered heavy exploitation, with a significant reduction in their biomass.
Bioenergetic traits of three keystone marine species in the food web of a pristine Patagonian fjord
The Patagonian fjords are high-latitude aquatic ecosystems, highly sensitive to climate change and play a key role in the exchange of organic matter and carbon flows between terrestrial and marine environments. The bioenergetic composition of species living in these ecosystems are fundamental to understanding the distribution, seasonal variations, and exchange of organic matter within benthic communities. This study reports on the bioenergetic characteristics (lipids, protein, glucose, and energy content) of three keystone species with different life-style and feeding habits: a benthic sea star (Ctenodiscus australis); squat lobster (Munida gregaria); and a Patagonian notothenioid (Eleginops maclovinus). Samples were obtained from the Yendegaia Fjord (54°40'S - 68°50′W) in Chilean Patagonia. Our results indicate that M. gregaria has higher concentrations of lipids, proteins, glucose, and total energy compared to either E. maclovinus or C. australis. The predominance of lipids in all species is possibly related to physiological characteristics and feeding strategies. Also, may be associated with the availability of food and environmental conditions typical of a fjord ecosystem and the reproductive stage in that they were collected. These results suggest that marine animals inhabiting glacially influenced environments with low temperature and low productivity, requires a convergent physiological strategy characterized by high levels of energy storage (i.e. lipids) for metabolism and key bioenergetic processes such as growth and reproduction.