Dr. Urzua-Osorio, Angel

2018, Urzua-Osorio, Angel, Bascur Bascur, Miguel Ángel, Guzmán Rivas, Fabián, Urbina, Mauricio

Hemigrapsus crenulatus is a key species of coastal and estuarine ecosystems in the Southeastern Pacific and New Zealand. Since the gravid females-and their embryos-develop under conditions of variable salinity, we propose that low external salinity will be met with an increase in energy expenditures in order to maintain osmoregulation; subsequently, the use of energy reserves for reproduction will be affected. In this study, we investigate in H. crenulatus whether 1) the biomass and energy content of embryos is influenced by salinity experienced during oogenesis and embryogenesis and 2) how variation in the biomass and energy content of embryos affects larval energetic condition at hatching. Here at low salinity (5 PSU), egg-bearing females experienced massive and frequent egg losses, and therefore the development of their eggs during embryogenesis was not completed. In turn, at intermediate and high salinity (15 and 30 PSU) embryogenesis was completed, egg development was successful, and larvae were obtained. Consistently, larvae hatched from eggs produced and incubated at high salinity (30 PSU) were larger, had higher dry weight, and had increased carbon content and energy than larvae hatched from eggs produced at intermediate salinity (15 PSU). From these results, it is seen that the size and biomass of early life stages of H. crenulatus can be affected by environmental salinity experienced during oogenesis and embryogenesis, and this variation can then directly affect the energetic condition of offspring at birth. Therefore, this study reveals a “cascade effect” modulated by salinity during the early ontogeny.

2018, Viña, Natalia, Bascur Bascur, Miguel Ángel, Guzmán, Fabián, Riera, Rodrigo, Paschke, Kurt, Urzua-Osorio, Angel

Porcelain crabs inhabit from upper intertidal to subtidal habitats. These environments are characterized by highly variable environmental conditions, which subject species found in these habitats to stress. In this study, we compared reproductive traits of mothers [i.e. fecundity, reproductive output (RO), dry weight, organic matter] and physiological parameters of their offspring (i.e. wet weight, water content, dry weight, organic matter, lactate content of embryos) of three species of porcelain crabs that inhabit the Southeastern Pacific: Petrolisthes laevigatus (upper intertidal); P. violaceus (low intertidal); Allopetrolisthes punctatus (subtidal). Overall, female P. laevigatus had lower fecundity (802 ± 115 vs. 4181 ± 1097 embryos) and amount of organic matter in their embryo masses (0.053 ± 0.006 vs. 0.27 ± 0.025 g) but higher RO values (1.34 ± 0.34 vs. 0.20 ± 0.07) than Allopetrolisthes punctatus. In addition, P. laevigatus embryos had higher organic matter content (81.09 ± 28.8 vs. 64.54 ± 6.1 μg), higher water content (188.6 ± 91.9 vs. 152.4 ± 30.8 μL) and higher lactate content (0.26 ± 0.04% vs. 0.07 ± 0.01% dry weight) than that found in A. punctatus embryos. Furthermore, females and embryos of P. violaceus showed low values and similar to those observed in P. laevigatus. As a potential strategy to increase survival of the offspring, P. laevigatus seems to invest a large portion of its energy in production of high quality embryos, despite costs to fecundity. This study reveals that porcelain crabs have physiological adaptations during their ontogeny that allow them to survive in fluctuating environments.