Dr. George-Nascimento-Failla, Mario

Notothen fishes (Nototheniidae) have been recorded on the coasts of South America, from 35°S to southern. Little is known about the biology of intertidal notothen fishes or their relationships with other species, including parasites. Here, we analyzed the parasitic fauna of a species of Notothenia collected from the rocky intertidal of Lebu, south-central Chile (36°S) and compared the parasite community of this host with other 9 fish species commonly found in rocky intertidal pools. Among the tested species, Notothenia sp. had the highest parasite richness (21 parasitic taxa vs. 1 to 9 parasitic taxa). The composition of parasite species was different among fishes, with similarity indices between 0 and 33%. The parasite composition of Notothenia sp. was most similar to Calliclinus geniguttatus (27%). The most abundant and prevalent parasites found in Notothenia sp. were the copepod Caligus cf. cheilodactyli, Tetraphyllidea larvae, anisakid nematodes, and several acanthocephalan species, which are typical of other fish from the subtidal and bento-demersal zones. Therefore, we conclude that the juveniles of Notothenia sp. were a few months old and had their parasites when reaching the intertidal zone, acquired some generalist parasites during their stay in this habitat, such as Holobomolochus chilensis and Lecithaster macrocotyle. Most notothen fishes are from Antarctic and Sub-Antarctic waters, therefore, it is possible that this fish came from another habitat but also from the austral region of South America. Notothenia sp. could not be specifically identified because of different results between morphological and genetic analyses.

The most studied digenean of marine organisms in Chile is by far Proctoeces humboldti, a parasite of the intestine of the clingfish Sicyases sanguineus and gonad of the keyhole limpet Fissurella spp. (progenetic metacercariae). The mussel Perumytilus purpuratus has been suggested as the first intermediate host for this digenean. In a study examining the parasites of S. sanguineus from central Chile, we found specimens of Proctoeces showing significant morphological differences with P. humboldti. To assist in the resolution of the taxonomic identification of these specimens, as well sporocysts obtained from the mussel P. purpuratus from central and northern Chile, phylogenetic studies using DNA sequences from the SSU rRNA, as well the LSU rRNA and Cox 1 gene were performed. Results showed that the clingfish S. sanguineus is a host for two species of Proctoeces (P. humboldti and P. syciases n. sp.) along the northern and central Chilean coast, without geographic separation; the mussel P. purpuratus is the first intermediate host for P. syciases n. sp. but not for P. humboldti in central and northern Chile. Fissurellids (Archaeogastropoda) along the Chilean coast harbor only progenetic stages of P. humboldti, but there is no evidence of progenesis for P. syciases. The reinstatement of Proctoeces humboldti is strongly suggested.

The coasts of central Chile and central California are important points of comparison in the study of ecological convergence such as a host’s parasite load because of their similar environmental conditions and the shared presence of many families of different species. In this study, the diversity of parasites in fish species from both zones was analyzed and compared to establish if there are similarities between them. The presence of 6 taxonomic groups of parasites was determined using published literature and databases for each location. A presence-absence matrix was created for the fish species studied in Chile and California, and a similarity analysis was carried out to prove whether the parasite loads of both zones were similar. The parasite taxa most frequently found in fish in central California were Digenea and Nematoda, whereas in central Chile the common taxa were Digenea, Annelida, Copepoda, Acanthocephala, and Nematoda. The similarity analysis showed that the parasite composition was different between zones. Nevertheless, overlaps were obtained in the parasitic diversity grouping the host in 3 host groups, one of which consists of hosts from both zones. This difference can be explained by the low parasitic diversity in the assemble of rocky intertidal fishes in California, potentially due to the limited amount of existing studies on intertidal fish parasites in California, along with other possible factors not explored in the present study.

Two new digenean species are described from the intertidal blenny Scartichthys viridis (Valenciennes) (Blenniidae) collected off the coasts of Chile. The digenean Monorchimacradena viridis n. sp. (Lecithasteridae: Macradenininae) differs from the only known species described in the genus, M. acanthuri Nahhas and Cable 1964, in the presence of Drüsenmagen in the caeca, the location of the seminal vesicle between the testis and ovary (anterior to the testis in M. viridis n. sp.), and the pre-ovarian vitellarium. Megasolena littoralis n. sp. (Haploporidae), which is also reported from Scartichthys gigas (Steindachner), differs from the five valid species of Megasolena in that the post-caecal region (from the posterior edge of the caeca to the end of the body) is larger in M. viridis n. sp., meaning that the caeca are shortest in this species. Also, M. littoralis n. sp. is distinguished, from the other congeneric species, in a combination of characteristics, e.g., body length, suckers, pharynx, testes, hermaphroditic sac and sucker-length ratio. Sequences of ITS2 rDNA were generated for specimens of both species from various localities, indicating that it was invariant within the species. This study describes species of Monorchimacradena and Megasolena in Chilean waters, here reported for the first time off the Pacific Coast of South America.

It was assessed the parasitic relationship between by 2 morphospecies of microphallid metacercariae and their second intermediate host, the amphipod Apohyale hirtipalma with the body length and sex of the hosts, and the abundance of shore birds (definitive hosts). Amphipod mortality induced by the parasites was assessed through the relationship between the variance/mean parasite abundance ratio with host body length for each metacercariae morphospecies. Four sites at the Biobío Region (36°S), Chile, were selected because of contrasting abundance of potential definitive hosts (Aves: Laridae), from where 240 amphipods were collected from intertidal pools (60 in each sampling locality). Total prevalence was 57.5% and metacercariae found, belonged to 2 unidentified morphospecies of microphallid subfamilies (Microphallinae, 28.3% and Maritrematinae, 36.7%). The prevalence and abundance of metacercariae increased with the amphipod body, although their variations were associated to the abundance of shorebirds. The prevalence of Microphallinae did not have differences among sexes in their relationship with the host body length, while among Maritrematinae, male amphipods showed higher prevalence than females of similar body length. It was also determined that Maritrematinae would cause more mortality than Microphallinae. Local variations of the abundance of other hosts involved in the microphallid life cycles as well as of prevailing local environmental conditions and amphipod behavior may be keys for a better understanding of the abundance and prevalence variations of these parasites.

A pesar que la interacción entre parásitos y hospederos es constante, y obligatoria para los parásitos, las variaciones espaciales de estas relaciones son poco conocidas. En este estudio se cuantifica y compara las interacciones parásito-hospedero en peces de pozas intermareales de la zona centro (33°S) y centrosur (36°S) de Chile. Se recolectó un total de 2.081 peces desde 56 pozas intermareales. Se comparó la biomasa y abundancia de peces entre las pozas de las dos zonas: centro (ZC) y centro-sur (ZCS). Para el análisis de las comunidades de parásitos se consideraron especies de peces comunes entre la ZC y ZCS, y se comparó la prevalencia, abundancia, riqueza y diversidad de parásitos. Tanto la abundancia y biomasa de peces como los parámetros comunitarios de parásitos, fueron estandarizados al volumen de la poza (m3). Pese a que hubo diferencias, entre ambas zonas, en la abundancia y biomasa en algunas especies de peces y en al menos un parámetro comunitario de parásitos, esas diferencias desaparecieron luego de estandarizar los datos a m3. Se detectó una media-baja similitud de especies parásitas entre zonas de muestreo (<55%). Sin embargo, los parámetros de interacción parásito-hospedero (conectancia y densidad de vínculos) no fueron distintos. Esto se puede deber, en parte, a la similitud en la riqueza de especies de peces, independiente de la especie de parásito, que mantendría su “carga de especies parásitas”. Las comunidades de parásitos de peces se han determinado como insaturadas, lo que significa que no tienen todas las especies posibles que pueden albergar, por lo que quedarían microhábitats disponibles en los hospederos para ser ocupados por especies parásitas propias de un lugar.

A new species, Hemipera cribbi sp. nov., is described. This trematode was found in three intertidal fish species: Scartichthys viridis (Valenciennes) (Blenniidae), Gobiesox marmoratus Jenyns (Gobiesocidae) and Myxodes viridis Valenciennes (Clinidae) from the central and southern coast of Chile. Of 233 individuals of S. viridis from the central coast examined, 19 were infected. From the southern coast, nine individuals of S. viridis (one infected), five individuals of G. marmoratus (four infected), and 16 individuals of M. viridis (one fish infected) were examined. Hemipera cribbi sp. nov. is distinguished from the five other congeneric species mainly in the body size, being the smallest and narrowest species in the genus, reaching five times longer than wide. This is the first species of the genus described for the South Pacific Ocean off South America. ITS2 rDNA sequences of Hemipera cribbi sp. nov. from each host and locality were identified.

Los Digenea son platelmintos parásitos comunes en organismos intermareales, y en sus etapas de vida libre (miracidio y cercaria) están expuestos a distintas condiciones ambientales, al igual que sus hospedadores. Por otro lado, éstos últimos pueden presentar un comportamiento fisiológico diferente si están o no parasitados. En este estudio se evaluó el efecto de la parasitosis por un digeneo, aún no identificado, sobre el consumo de oxígeno del chorito Perumytilus purpuratus, y se analizó la influencia de la temperatura del agua y los ciclos de emersión-inmersión sobre la liberación de las cercarias de estos parásitos. Ejemplares de P. purpuratus fueron recolectaron manualmente desde el roquerío intermareal de la desembocadura del río Biobío, Chile. En el laboratorio se comparó, en experimentos con distintos ejemplares, el consumo de oxígeno de individuos con y sin parásitos, y se midió la liberación de cercarias a 9, 17 y 22°C de temperatura del agua, y según ciclos opuestos de inmersión-emersión de duración variable. El consumo de oxígeno no mostró diferencias significativas entre choritos parasitados y no parasitados. Sin embargo, los ejemplares parasitados mostraron escaso desarrollo gonadal, lo que sugiere una reasignación de los productos metabólicos de los choritos hacia el parásito. La liberación de cercarias aumentó con la temperatura del agua y decreció exponencialmente con la duración de la inmersión-emersión, alcanzando valores máximos en las primeras 2 h de ocurrida la inmersión. Las secuencias opuestas de los ciclos de inmersión-emersión empleadas en los experimentos no influyeron significativamente en el número final de cercarias liberadas. Estos resultados sugieren que el metabolismo del hospedador aumenta con la temperatura, y que la inmersión y la temperatura del agua inducen la liberación de las cercarias.

Eumetazoan parasites in fish larvae normally exhibit large body sizes relative to their hosts. This observation raises a question about the potential effects that parasites might have on small fish. We indirectly evaluated this question using energetic metabolic laws based on body volume and the parasite densities. We compared the biovolume as well as the numeric and volumetric densities of parasites over the host body volume of larval and juvenile-adult fish and the average of these parasitological descriptors for castrator parasites and the parasites found in the fish studied here. We collected 5266 fish larvae using nearshore zooplankton sampling and 1556 juveniles and adult fish from intertidal rocky pools in central Chile. We considered only the parasitized hosts: 482 fish larvae and 629 juvenile-adult fish. We obtained 31 fish species; 14 species were in both plankton and intertidal zones. Fish larvae exhibited a significantly smaller biovolume but larger numeric and volumetric densities of parasites than juvenile-adult fish. Therefore, fish larvae showed a large proportion of parasite biovolume per unit of body host (cm3). However, the general scaling of parasitological descriptors and host body volume were similar between larvae and juvenile-adult fish. The ratio between the biovolume of parasites and the host body volume in fish larvae was similar to the proportion observed in castrator parasites. Furthermore, the ratios were different from those of juvenile-adult fish, which suggests that the presence of parasites implies a high energetic cost for fish larvae that would diminish the fitness of these small hosts.

Research using parasites infish population studies in the South Eastern Pacific (SEP) is summarized. There are 27 suchstudies (snapshots mainly) in single host species sampled at different geographic localities and at somewhat similar times.They have been devoted mainly to economically important species, though others on coastal and intertidalfish or on less- ornon-commercial species provide insights on scales of temporal and spatial variation of parasite infracommunities. Later, weassess whether the probability of harbouring parasites depends on the host species body size. Our results indicate that astronger tool forfish population studies may be developed under regular (long term) scrutiny of parasite communities,especially of smallfish host species, due to their larger variability in richness, abundance and total biomass, than in largefishspecies. Finally, it might also be necessary to consider the effects offishing on parasite communities as well as the naturaloscillations (coupled or not) of host and parasite populations.