Dr. George-Nascimento-Failla, Mario

2020, Aldana, M., Pulgar, J., Hernández, B., George-Nascimento-Failla, Mario, Lagos, N. A., García Huidobro, M. R.

Parasites alter the reproductive performance of their hosts, limit their growth, and thereby modify the energy budget of these hosts. Experimental studies and theoretical models suggest that the outcome of the host-parasite interactions could be determined by ecological factors such as food availability levels in the local habitats. Nutrient inputs may affect the host's food resource availability with positive or negative effects on parasite infection rates and tolerance of infection, however this has not been specifically evaluated in natural systems. In this study, we evaluate the effects of parasitism by Proctoeces humboldti on body size, gonadosomatic index (GSI), and metabolic rate (oxygen consumption) of their second intermediate host Fissurella crassa limpets, under contrasting natural conditions of productivity (upwelling center vs upwelling shadow sites). Our results evidenced that parasitized limpets collected from the intertidal habitat influenced by coastal upwelling site showed greater shell length, muscular foot biomass and GSI as compared to non-parasitized limpets collected in the same site, and compared to parasitized and non-parasitized limpets collected from the sites under the influence of upwelling shadow conditions. Oxygen consumption was lower in parasitized limpets collected from the upwelling-influenced site than in the other groups, independent of age, suggesting reduced metabolic stress in infected individuals inhabiting these productive sites. Our results suggest that increased productivity in upwelling sites could mitigate the conflict for resources in the P. humboldti – F. crassa system, influencing where such interaction is found in the continuum between parasitism and mutualism. Since parasitism is ubiquitous in natural systems, and play important roles in ecological and evolutionary processes, it is important to analyze host-parasite interaction across a variety of ecological conditions, especially in biological conservation.

2021, López Rodríguez, Ruby, George-Nascimento-Failla, Mario, Górski, Konrad

Diplostomatid digeneans are well-known manipulators of the behavior of their intermediate hosts. Unencysted metacercariae of Tylodelphys sp. inhabit the cranial cavity of the fish Galaxias maculatus; however, to date they have not been documented to alter their host behavior. The goal of this study was to evaluate the potential effects of Tylodelphys sp. inhabiting the cranial cavity of Galaxias maculatus on host physiology and swimming behavior as well as its reaction to a simulated predation attempt. Blind experiments in the lab were carried out on 56 fish that were filmed individually. The Fulton condition factor (K) was used as an approximation of nutritional status and a respirometry chamber was used to evaluate oxygen consumption rates of fish. Of the 56 fish, 21 were parasitized by Tylodelphys sp. (mean intensity = 30, range from 1 to 101). Parasitized and non-parasitized fish were similar in condition factor and oxygen consumption rates. Furthermore, the oxygen consumption rate of G. maculatus was not correlated with the abundance of Tylodelphys sp. However, parasitized fish more frequently swam close to the water surface, whereas non-parasitized fish more frequently swam at intermediate depths. When faced with a simulated predator attack, unparasitized fish showed more frequent fleeing behavior as well as a more intense post-fleeing activity. Collectively, these results suggest that Tylodelphys sp. inhabiting the cranial cavity of fish may alter their behavior predisposing them to predation by birds.

2024, Lugo-Pérez, Luisana, Vera-Escalona, Iván, Dr. George-Nascimento-Failla, Mario, Dr. Brante-Ramirez, Antonio, Dra. Gamboa-Mendez, Maribet

Las especies del género Anisakis (Nematoda: Anisakidae) son parásitos marinos con ciclo de vida indirecto. Los crustáceos planctónicos actúan como primeros hospedadores intermediarios, mientras que peces y cefalópodos intervienen como segundos hospedadores intermediarios o paraténicos, finalmente el ciclo se cierra en los cetáceos, mamíferos marinos que son los principales hospedadores definitivos del género. En el ciclo de vida, las larvas de Anisakis pueden ser ingeridas por el hombre interviniendo como huésped accidental, lo que puede ocasionar anisakiasis, una zoonosis adquirida a través del consumo de peces y cefalópodos crudos o marinados. Estos nemátodos tienen una distribución cosmopolita, sin embargo, su diversidad ha sido escasamente estudiada en el hemisferio Sur. Por tanto, se evaluó la diversidad de las larvas de Anisakis spp., presentes en dos especies de hospederos de Chile, combinando el análisis morfométrico y genético. Para ello, se recolectaron larvas de Anisakis spp. en la cavidad abdominal de la merluza Merluccius gayi y el calamar Dosidicus gigas, procedentes de terminales pesqueros de la región del Biobío, Chile. La caracterización morfométrica de las larvas de Anisakis spp., consistió en la medición de la longitud del esófago, ventrículo esofágico, cola, longitud total y ancho máximo del cuerpo. Para los análisis genéticos se usó la región molecular nuclear ITS (ITS1-ITS2) y mitocondrial COX2. Los resultados morfométricos revelaron que las larvas extraídas de D. gigas son significativamente de mayor longitud que las recolectadas en M. gayi, sugiriendo una alta variabilidad fenotípica hospedador-dependiente. Los análisis moleculares y filogenéticos determinaron la presencia de Anisakis pegreffii en ambos hospedadores, sin embargo, demostraron una baja diferenciación genética y diversidad nucleotídica entre las secuencias, indicando una escasa variabilidad genética para el conjunto de datos. Este trabajo constituye el primer registro molecular de A. pegreffii en hospedadores intermediario o paraténicos de la costa de Chile.

2021, Dr. George-Nascimento-Failla, Mario, Rodríguez, Sara

The scarce information on the helminth fauna in otariids from the Southeastern Pacific comes mainly from stranded individuals or killed for that purpose. In this study, we compared the abundance and composition of enteroparasitic assemblages of Otaria flavescens using coprological techniques. Three sampling localities from north to south spanning 2,200 km off the Chilean coast were considered (Iquique, Viñ a del Mar, and Talcahuano). In all, 60 fecal samples were collected, and eggs belonging to 5 taxa were found in 91.6% of the samples. They were the anisakid nematodes Contracaecum and Pseudoterranova, the cestode Adenocephalus (syn. Diphyllobothrium), the trematode Ogmogaster, and the acanthocephalan Corynosoma. Samples from southern Chile (Talcahuano) showed the highest prevalence. Adenocephalus eggs had the highest prevalence and abundance in Iquique and Talcahuano, whereas Ogmogaster was the less prevalent and abundant in all sampling localities. Corynosoma eggs had similar prevalence and abundance among sampling localities, and Pseudoterranova eggs were absent in Iquique and with median prevalence values in Viñ a del Mar and Talcahuano. Thus, the composition of parasite egg assemblages was different between sampling localities. These differences between sampling localities may help to explain differential records of some zoonotic parasitoses such as pseudoterranovosis and diphyllobothriosis in Peru and Chile, where consumption of raw or marinated fish (ceviche) is common. For example, the lower diversity of parasite egg assemblages in the northern Chilean coast may be due to the absence or lower abundance of first intermediate/paratenic hosts of Pseudoterranova.

2020, George-Nascimento-Failla, Mario, López-Rodríguez, Ruby, Górski, Konrad

Galaxias maculatus is an abundant freshwater fish species in Chilean continental waters where it plays important ecological functions, yet few parasitological records of this species exist in Chile and all of them cover a very limited geographic range. The objective of this study was to assess large scale geographic variation in composition of parasite infracommunities of Galaxias maculatus. Specifically, parasite infracommunities of this species were compared among 11 locations across 9 degrees of latitude and 3 ecosystem types (lake, river and estuary). Most taxa found had been previously reported in Chile and Argentina. However, this is the first report for Tylodelphys sp. in this host in Chile. Furthermore, the cranial parasite Tylodelphys sp. had the highest overall prevalence and abundance compared to other parasite species. Despite the fact that the abundance of Tylodelphys sp. was not significantly correlated with Fulton’s condition factor of fish, infected fish seem to have a better body condition compared to uninfected ones. The most important source of variation in composition of infracommunities was the sampling location. Furthermore, fish from lakes have a different composition of parasite infracommunities mainly due to higher abundances of Tylodelphys sp.

2020, González, M. T., López, Z., Núñez, J. J., Calderón-Mayo, K. I., Ramírez, C., Morgades, D., Katz, H., George-Nascimento-Failla, Mario, Pavés, H.

Hookworms of the genus Uncinaria parasitize pinniped pups in various locations worldwide. Four species have been described, two of which parasitize pinniped pups in the southern hemisphere: Uncinaria hamiltoni parasitizes Otaria flavescens and Arctocephalus australis from the South American coast, and Uncinaria sanguinis parasitizes Neophoca cinerea from the Australian coast. However, their geographical ranges and host specificity are unknown. Uncinaria spp. are morphologically similar, but molecular analyses have allowed the recognition of new species in the genus Uncinaria. We used nuclear genetic markers (internal transcribed spacer (ITS) and large subunit (LSU) rDNA) and a mitochondrial genetic marker (cytochrome c oxidase subunit I (COI)) to evaluate the phylogenetic relationships of Uncinaria spp. parasitizing A. australis and O. flavescens from South American coasts (Atlantic and Pacific coasts). We compared our sequences with published Uncinaria sequences. A Generalized Mixed Yule Coalescent (GMYC) analysis was also used to delimit species, and principal component analysis was used to compare morphometry among Uncinaria specimens. Parasites were sampled from A. australis from Peru (12°S), southern Chile (42°S), and the Uruguayan coast, and from O. flavescens from northern Chile (24°S) and the Uruguayan coast. Morphometric differences were observed between Uncinaria specimens from both South American coasts and between Uncinaria specimens from A. australis in Peru and southern Chile. Phylogenetic and GMYC analyses suggest that south-eastern Pacific otariid species harbour U. hamiltoni and an undescribed putative species of Uncinaria. However, more samples from A. australis and O. flavescens are necessary to understand the phylogenetic patterns of Uncinaria spp. across the South Pacific.