Research Outputs
Variability in the energy reserves of swordfish (Xiphias gladius) of the southeastern Pacific Ocean: A temporal and intra-individual perspective
The temporal dynamics of energy reserves are associated with the physiological processes (i.e., reproduction) in marine fishes, in which storage organs play a key role for efficient energy investment. We evaluated the temporal (i.e., seasons) and intra-individual (i.e., organs) dynamics of adult female swordfish (Xiphias gladius) during its feeding period off the Chilean coast in the southeastern Pacific Ocean (SEPO). The biochemical composition (i.e., lipids, proteins, and glucose), energy content and fatty acid profile of the muscle, liver and gonad were evaluated during the austral autumn, winter, and spring. Our results showed principally an intra-individual effect in both the muscle and liver in the autumn and spring. Herein, a trend of higher amounts of lipids and total energy were found in the muscle, while the liver showed greater protein and glucose contents. Consequently, the muscle showed a higher saturated, monounsaturated, and polyunsaturated fatty acid contents than the liver. Although the gonad showed no significant temporal effect in the lipids and proteins contents, an increasing trend of each biochemical constituent, fatty acid group and gonadosomatic index were found from autumn to winter. Consistently, the glucose and total energy content as well Fulton's condition factor were significantly higher in winter. These findings reflect the spatial-temporal physiological dynamic of swordfish based on the storage of energy reserves in different organs during its feeding period. In this way, the products obtained from swordfish could have an added value depending on the season and capture zone, which could benefit the exploitation and regulation measures of this resource under an ecological approach of conservation and sustainability in the SEPO.
Consumption of marine-derived nutrients from invasive Chinook salmon (Oncorhynchus tshawytscha) transfer ω-3 highly unsaturated fatty acids to invasive resident rainbow trout (O. mykiss)
Marine-derived nutrients (MDN) contained in gametes (mature eggs and sperm), carcasses and metabolic wastes from anadromous migratory salmon can transfer energy and materials to fresh water, thereby affecting the structure and function of stream ecosystems. This is crucial among ecosystems where humans have mediated biological invasions by propagating non-native species. Previous studies have demonstrated that consumption of MDN from salmon can benefit both native and invasive resident fishes. Yet, a more detailed understanding of the transfer of biomolecules with important physiological functions such as ω-3 highly unsaturated fatty acids (HUFAs) have received less attention among researchers. Here we demonstrate that consumption of MDN contained in invasive Chinook salmon eggs transfers ω-3 HUFAs (e.g., EPA and DHA) to resident invasive rainbow trout in a river food web. We conducted a field study in river sections previously identified as spawning areas for Chinook salmon in the Cisnes River, Patagonia. Rainbow trout were sampled around salmon spawning areas before, during, and after the salmon spawning season. Additionally, we collected tissue from different food web resources and components of different origin (e.g., primary producers, aquatic and terrestrial items) from the Cisnes River system. Analyses of stomach contents of trout were performed in conjunction with analyses of both lipid content and fatty acid profiles of trout tissue and food web components. Chinook salmon eggs showed higher content of ω-3 HUFAs, especially EPA (31.08 ± 23.08 mg g DW−1) and DHA (27.50 ± 14.11 mg g DW−1) than either freshwater or terrestrial components (0–6.10 mg g DW−1 both EPA and DHA). We detected marked shifts in the fatty acid profile (~six-fold increase in EPA and DHA) of trout following consumption of Chinook salmon eggs. Our findings suggest that MDN via consumption of salmon eggs by resident rainbow trout may positively influence resident trout and likely contribute to gauge synergistic interactions between invaders on receiving ecosystems of Patagonia.
Spatio-temporal changes in the biochemical parameters of the fishery resource Concholepas concholepas (Gastropoda: Muricidae) in the Southeastern Pacific Ocean
The fishery resource Concholepas concholepas is a key species in the benthic marine ecosystems of the Southeastern Pacific Ocean. In the present study, the spatio-temporal changes in the biochemical parameters were evaluated for adult individuals of C. concholepas. They were sampled in summer and winter under upwelling and Non-upwelling conditions in two Management and Exploitation Areas for Benthic Resources of Valparaíso, Chile. The results indicated that the variation in the biochemical parameters is explained to a greater extent by the season. For example, in both upwelling and Nonupwelling conditions, we found a higher content of total lipids, proteins, glucose, and energy in winter than summer. Temporal variations can be explained by the reproductive process after summer season, in which the egg spawning require a greater energy reserves to be perform. Nonetheless, differences in the nutritional status of preys along seasons of the year, can also influence on these results. In fact, these variations in the biochemical parameters of C. concholepas may be indicative of an adaptive seasonal physiological response to the environment for maintaining an optimal energy budget year-round. Altogether, this knowledge will contribute to the Management and Exploitation Areas for Benthic Resources, improving regulatory measures during the annual period of captures and landings of C. concholepas, favoring a more sustainable fishery in the Southeastern Pacific Ocean within an ecosystem approach.
The cascade of effects caused by emersion during early ontogeny in porcelain crabs of the Southeast Pacific coast: Biochemical responses of offspring
Petrolisthes crabs inhabit a wide range of coastal environments, from the upper intertidal to the subtidal, experiencing regular changes in pH, salinity, and temperature. Hence, such subtidal and intertidal invertebrates are likely to show physiological and biochemical adaptive responses in order to successfully develop during early ontogenetic stages and thus reach reproduction. We herein evaluated the biochemical responses to contrasting environmental conditions of the early ontogenetic stages of two coastal crabs from the Southeast Pacific coast: Petrolisthes laevigatus and Allopetrolisthes punctatus. For this purpose, stage I embryos of both species were subjected to two treatments: (1) emersion (i.e., a daily 3 h aerial exposure until the zoeas hatched) and (2) immersion (i.e., uninterrupted underwater submersion until the zoeas hatched); the total contents of glucose, proteins, lipids, and fatty acids of the organisms were measured in stage I embryos and recently hatched zoeas in order to assess the biochemical constitution of the two species. Both species showed changes in their energetic reserves when treatments within species were compared. Our results found that A. punctatus was negatively affected by stressful periods of emersion, while P. laevigatus showed the opposite tendency and was affected by periods of immersion. The sensitivity of the response and the contrasting outcomes for these two crabs underpin the fact that changes in environmental conditions along the Chilean coast due to climate change (e.g., increased anoxic coastal waters) may have significantly negative consequences on the populations of these ecologically important species and the associated taxa within their ecosystems.
Host-parasite dialogue: Fecundity compensation mechanisms of Fissurella crassa
Parasites can alter the reproductive performance of their hosts, and to avoid or mitigate the resulting fitness loss, hosts may increase their current reproductive output to compensate for the future loss due to the parasitic infection. Fecundity compensation can be exploited by parasites for their own transmission (exploitation of host compensatory responses by parasites). However, this phenomenon has rarely been reported in second intermediate hosts of trematodes and its mechanisms and consequences largely unexplored. Along the east coast of the South Pacific, the second intermediate host, the mollusk Fissurella crassa, has been observed to display higher muscular foot, greater shell length and weight, and a higher gonadosomatic index when parasitized by metacercariaes of Proctoeces humboldti compared to non-parasitized hosts. In this study, we examined the histology, biochemistry (glucose, lipids, and proteins), and levels of sex hormones (estradiol and progesterone) in both parasitized and non-parasitized female individuals of F. crassa. Our findings revealed that the gonad of parasitized limpets had a higher density of oocytes, but these had a smaller individual area. Additionally, the gonadal tissue of parasitized limpets exhibited lower glucose content but higher lipid content. Notably, the levels of progesterone increased with parasite intensity. These results suggest that F. crassa possesses the ability to compensate for the negative effects of parasites by increasing the number of oocytes through biochemical and hormonal mechanisms. Our study contributes to the limited research on the impact of metacercariae on the reproduction of second intermediate hosts. Furthermore, we discuss how these changes in parasitized limpets could benefit parasite transmission.
Seasonal variation in reproductive parameters of the squat lobster Pleuroncodes monodon from a South Pacific population
Pleuroncodes monodon from the Chilean coast has seasonal reproduction. During the seasonal period, females and their eggs are exposed to seasonal variation in environmental conditions. In P. monodon populations inhabiting the Southern Pacific coast near Concepción, Chile, we quantified late summer (February–March 2014) and late winter (August–September 2014) seasonal variations in female reproductive parameters (carapace length, fecundity, reproductive output (RO), ash weight, organic content) as well as eggs parameters (length, volume, dry weight, organic content). There was conspicuous seasonal variation in the main reproductive parameters of this species. During the summer, the number of eggs and organic content of females were higher than winter, whereas RO was slightly higher in winter than in summer. Significant variation in the size and biomass of the eggs was also found between seasons. For example, eggs laid in summer were smaller, had a lower measured dry mass, and had a lower organic content than eggs laid in winter. Seasonal variations in the fecundity and size of the eggs have implications for fishery models, which can be used to estimate the relative contribution of different cohorts to recruitment and stability of adult benthic populations.
Temporal and inter-individual changes in the integrated biochemical condition of the gonads of female swordfish (Xiphias gladius) from the Southeastern Pacific Ocean
The integrated biochemical condition (IBC) of gonads is closely related to the reproductive success of highly migratory marine species. The IBC of gonads can be influenced not only by size and/or age, but also by environmental conditions. Here, female swordfish, Xiphias gladius, that migrate to temperate regions with a marked seasonality (e.g., the Southeastern Pacific Ocean, SEPO) were compared in relation to the IBCs (lipids, proteins, glucose and, fatty acid profiles) of their gonads; individuals with two body size ranges and distinct degrees of sexual maturity were evaluated, and considered as: small and/or virginal (SV: <170 cm lower jaw fork-length (LJFL), oocyte size (OS) <0.08 mm) vs large and/or maturing females (LM: >190 cm LJFL, OS >0.133 mm). This comparison was conducted in two environmentally contrasting seasons (winter vs spring). Our results showed that the gonadosomatic index (GSI) was significantly higher in LM than SV. Lipid contents varied significantly between seasons and body sizes. The highest lipid concentrations were recorded in the spring in large females. No significant differences were found when comparing the protein and glucose contents of the two evaluated seasons or body size ranges of the studied females. In turn, the fatty acid (FA) profiles of female gonads significantly varied for both seasons and body size ranges. A high content of saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs) and poly-unsaturated fatty acids (PUFAs) were recorded in female gonads in the spring. The SFAs C16:0 and C18:0, the MUFA C18:1n9, and the essential PUFA C22:6n3 were the main contributors to the observed differences between spring and winter. These results could be used as indicators of the nutritional condition and health status of swordfish individuals. Hence, the IBC of female swordfish gonads have great potential to aid in estimating survival rates and stock abundances of this species. The integration of this information constitutes an asset in fishery management models with an ecosystem approach.
Revealing coastal upwelling impact on the muscle growth of an intertidal fish
Upwelling oceanographic phenomenon is associated with increased food availability, low seawater temperature and pH. These conditions could significantly affect food quality and, in consequence, the growth of marine species. One of the most important organismal traits is somatic growth, which is highly related to skeletal muscle. In fish, skeletal muscle growth is highly influenced by environmental factors (i.e. temperature and nutrient availability) that showed differences between upwelling and downwelling zones. Nevertheless, there are no available field studies regarding the impact of those conditions on fish muscle physiology. This work aimed to evaluate the muscle fibers size, protein content, gene expression of growth and atrophy-related genes in fish sampled from upwelling and downwelling zones. Seawater and fish food items (seaweeds) samples were collected from upwelling and downwelling zones to determine the habitat's physical-chemical variations and the abundance of biomolecules in seaweed tissue. In addition, white skeletal muscle samples were collected from an intertidal fish to analyze muscular histology, the growth pathways of protein kinase B and the extracellular signal-regulated kinase; and the gene expression of growth- (insulin-like growth factor 1 and myosin heavy-chain) and atrophy-related genes (F-box only protein 32 and muscle RING-finger protein-1). Upwelling zones revealed higher nutrients in seawater and higher protein content in seaweed than samples from downwelling zones. Moreover, fish from upwelling zones presented a greater size of muscle fibers and protein content compared to downwelling fish, associated with lower protein ubiquitination and gene expression of F-box only protein 32. Our data indicate an attenuated use of proteins as energy source in upwelling conditions favoring protein synthesis and muscle growth. This report shed lights of how oceanographic conditions may modulate food quality and fish muscle physiology in an integrated way, with high implications for marine conservation and sustainable fisheries management.
Biochemical-ecological composition and bio-stoichiometric ratios of swordfish (Xiphias gladius) gonads in the Southeastern Pacific Ocean
Swordfish (Xiphias gladius), a highly migratory fishery resource of great importance in the Southeastern Pacific Ocean (SEPO), is a species with late sexual maturation, high longevity, and a large body size that spawns and reproduces several times throughout its adulthood. In the present study, we hypothesized that the Biochemical-Ecological Composition (BEC) of the gonad (measured as the lipid, protein, glucose, and energy contents) and its bio-stoichiometric ratios (lipid/protein: L/P; lipid/ glucose: L/G) would be influenced by both female body size (lower jaw fork length in cm, LJFL: <180, 180–260, >260) and time (trimesters: 1,2,3; years: 2017, 2019). Our results revealed that in relation to their body size, female swordfish gonads varied significantly in their BEC, but not in their L/P and L/G ratios during their quarterly development within an annual cycle. These variations in the BEC were noticeable in females of medium (180–260) and larger (>260) sizes. Particularly, as the year progressed, females stored significant amounts of energy during their trophic migration, made evident by the increase in the lipid and protein contents of the gonads, which reached maximum levels towards the end of the year, during the second and third trimesters. These high energy reserves can be used for the reproduction during the last trimester of the year (i.e. austral spring–summer), when the warm temperature and high productivity in the SEPO are optimal for the successful development of oocytes. Future studies considering an ecosystem approach should analyze the classes of lipids and types of fatty acids that are involved in the reproduction of this species, and how they are acquired through the consumption of lipid-rich prey, and subsequently metabolized to be used in reproduction and during early ontogeny.
Seasonal dynamics of biochemical composition and fatty acids of swordfish (Xiphias gladius) in the Southeast Pacific Ocean off the coast of Chile
In the Southeast Pacific Ocean, Xiphias gladius migrates through the Chilean coastal zone for feeding. Here, it forages for different prey items from autumn to spring, acquiring a great variety of energy and nutritional reserves. We evaluated seasonal variations in the biochemical reserves (i.e., contents of lipids, proteins, and glucose), total energy content and fatty acid profile of specimens captured during the austral autumn, winter, and spring. Our results show that higher amounts of lipids were found in the winter and spring, while protein and glucose were higher in the autumn. Thus, the energy content showed significant differences, with higher levels in winter and spring. Furthermore, the fatty acid profile was more diverse in the spring than the autumn and winter and was characterized by higher amounts of polyunsaturated fatty acids. These findings suggest that temporal changes in the biochemical reserves, total energy content and fatty acid profile support the idea of a “trophic migration” (i.e., the feeding period) established by the dynamics of fishery fleets. The high amounts of lipids and diverse fatty acid profile found in the spring could indicate the end of the trophic migration during this season. Thus, X. gladius may reach an optimum nutritional condition in the spring and make energetic adjustments to carry out its reproductive migration during the austral summer. Therefore, this species seems to meet the high energy demands of the reproductive season by foraging for a wide range of prey items from autumn to spring and storing an increased amount of lipids at the end of the feeding period. Overall, our data provides crucial baseline knowledge for future research on the ecophysiology of X. gladius, as well as for the management and conservation of this fishery resource under an ecosystem approach.