Research Outputs
The influence of glacial melt and retreat on the nutritional condition of the bivalve Nuculana inaequisculpta (Protobranchia: Nuculanidae) in the West Antarctic Peninsula
Due to climate change, numerous ice bodies have been lost in the West Antarctic Peninsula (WAP). As a consequence, deglaciation is expected to impact the marine environment and its biota at physiological and ecosystem levels. Nuculana inaequisculpta is a marine bivalve widely distributed around Antarctica that plays an important role for ecosystem functioning. Considering that N. inaequisculpta inhabits coastal areas under effect of glacial melt and retreat, impacts on its nutritional condition are expected due to alterations on its physiology and food availability. To test this hypothesis, biochemical composition (lipids, proteins, and fatty acids) and energy content were measured in individuals of N. inaequisculpta collected in a fjord at different distances to the retreating glacier in the WAP. Oceanographic parameters of the top and bottom-water layers (temperature, salinity, dissolved oxygen, and chlorophyll-a) were measured to investigate how the environment changes along the fjord. Results showed that surface oceanographic parameters displayed a lower temperature and dissolved oxygen, but a higher salinity and chlorophyll-a content at nearest compared to farthest sites to the glacier. In contrast, a lower temperature and chlorophyll-a, and a higher salinity and dissolved oxygen was measured in the bottom-water layer toward the glacier. N. inaequisculpta had a higher amount of lipids (17.42 ± 3.24 vs. 12.16 ± 3.46%), protein (24.34 ± 6.12 vs. 21.05 ± 2.46%) and energy content (50.57 ± 6.97 J vs. 39.14 ± 5.80 J) in the farthest compared to the nearest site to the glacier. No differences were found in total fatty acids among all sites. It seems likely that lower individual fitness related to proximity to the glacier would not be related to nutritional quality of sediment food, but rather to food quantity.
Host-parasite dialogue: Fecundity compensation mechanisms of Fissurella crassa
Parasites can alter the reproductive performance of their hosts, and to avoid or mitigate the resulting fitness loss, hosts may increase their current reproductive output to compensate for the future loss due to the parasitic infection. Fecundity compensation can be exploited by parasites for their own transmission (exploitation of host compensatory responses by parasites). However, this phenomenon has rarely been reported in second intermediate hosts of trematodes and its mechanisms and consequences largely unexplored. Along the east coast of the South Pacific, the second intermediate host, the mollusk Fissurella crassa, has been observed to display higher muscular foot, greater shell length and weight, and a higher gonadosomatic index when parasitized by metacercariaes of Proctoeces humboldti compared to non-parasitized hosts. In this study, we examined the histology, biochemistry (glucose, lipids, and proteins), and levels of sex hormones (estradiol and progesterone) in both parasitized and non-parasitized female individuals of F. crassa. Our findings revealed that the gonad of parasitized limpets had a higher density of oocytes, but these had a smaller individual area. Additionally, the gonadal tissue of parasitized limpets exhibited lower glucose content but higher lipid content. Notably, the levels of progesterone increased with parasite intensity. These results suggest that F. crassa possesses the ability to compensate for the negative effects of parasites by increasing the number of oocytes through biochemical and hormonal mechanisms. Our study contributes to the limited research on the impact of metacercariae on the reproduction of second intermediate hosts. Furthermore, we discuss how these changes in parasitized limpets could benefit parasite transmission.
Seasonal variation in reproductive parameters of the squat lobster Pleuroncodes monodon from a South Pacific population
Pleuroncodes monodon from the Chilean coast has seasonal reproduction. During the seasonal period, females and their eggs are exposed to seasonal variation in environmental conditions. In P. monodon populations inhabiting the Southern Pacific coast near Concepción, Chile, we quantified late summer (February–March 2014) and late winter (August–September 2014) seasonal variations in female reproductive parameters (carapace length, fecundity, reproductive output (RO), ash weight, organic content) as well as eggs parameters (length, volume, dry weight, organic content). There was conspicuous seasonal variation in the main reproductive parameters of this species. During the summer, the number of eggs and organic content of females were higher than winter, whereas RO was slightly higher in winter than in summer. Significant variation in the size and biomass of the eggs was also found between seasons. For example, eggs laid in summer were smaller, had a lower measured dry mass, and had a lower organic content than eggs laid in winter. Seasonal variations in the fecundity and size of the eggs have implications for fishery models, which can be used to estimate the relative contribution of different cohorts to recruitment and stability of adult benthic populations.
Fatty acid biomarkers in three species inhabiting a high latitude Patagonian fjord (Yendegaia Fjord, Chile)
The study of fatty acid biomarkers in trophic structures at sub-polar latitudes is fundamental in describing energy fluxes across ecosystems characterized by complex inter-specific interactions. Due to the presence of certain essential fatty acids obtained exclusively from predator–prey interactions, fatty acid biomarkers are widely used to identify trophic interactions. This study analyzed fatty acid compositions in three species inhabiting a relatively pristine Patagonian fjord. This fjord is geographically difficult to access, so there are very little sampling opportunities, biological and oceanographic information. In the three species collected (Ctenodiscus australis (Loven in Lütken 1871) (Echinodermata, Asteroidea, Ctenodiscidae); Munida gregaria (Fabricius 1793) (Arthropoda, Malacostraca, Munididae); Eleginops maclovinus (Cuvier 1830) (Chordata, Actinopterygii, Eleginopsidae)) along this remote area were evaluated their fatty acid trophic markers as a tool to differentiate dietary components and dietary habits. The study reported significant differences in the amount of saturated fatty acids (SFA), monounsaturated fatty acids (MUFA), and polyunsaturated fatty acids (PUFA), with the highest concentrations of all fatty acids in M. gregaria. The last suggests that M. gregaria is considered as a good quality food source or biological component that might support the fjord trophic web in the Southern Hemisphere. The results describe diet compositions in sampled species, and differences among species for fatty acid compositions and proportions. This provides an initial basis for future modeling or projecting how benthic ecosystems of fjords and Patagonian channels respond to food intake, particularly in environments associated with glacial systems characterized by a low phytoplankton biomass and greater sensitivity to climate variability.
Intra-individual variability in biochemical constituents and fatty acid composition of adult jumbo squid (Dosidicus gigas) in the southeastern Pacific Ocean
In marine invertebrates, the bioenergetic fuel available for fundamental physiological processes (growth, reproduction) may present intra-individual variability depending on the storage organ, sex and state of sexual maturity. This variability is considered relevant information for fishery management. In the squid Dosidicus gigas, an important fishery resource, we analyzed adult males (immature vs. mature) and females (immature I vs. immature II) off the coast of Chile. Their bioenergetic fuel (protein, glucose, lipid and fatty acid content-FAs) was quantified in different organs of the body (digestive gland, gonad and mantle). When comparing the organs of males at both stages of maturity, a greater amount of glucose and lipids were observed in the digestive gland than in the gonad and mantle, while a higher protein content was recorded in the gonad. In turn, the same tendency of biochemical variations among the organs was observed for the female at both stages. Regarding the FA profiles of the analyzed organs, the digestive gland had the highest mean proportion of FAs. However, no significant differences were observed related to sex and sexual maturity. According to the multivariate analyses for both sexes and maturity stages, the FA profiles of the mantle and gonad showed overlap and a high similarity, while the profile of the digestive gland was completely dissimilar. Our findings indicate that D. gigas from Chilean coastal waters showed, mainly in the digestive gland, high levels of all biochemical constituents, which are obtained through food and stored in their organs as bioenergetic fuel, and may then be used for the subsequent process of migration and reproduction in oceanic waters.
Revealing coastal upwelling impact on the muscle growth of an intertidal fish
Upwelling oceanographic phenomenon is associated with increased food availability, low seawater temperature and pH. These conditions could significantly affect food quality and, in consequence, the growth of marine species. One of the most important organismal traits is somatic growth, which is highly related to skeletal muscle. In fish, skeletal muscle growth is highly influenced by environmental factors (i.e. temperature and nutrient availability) that showed differences between upwelling and downwelling zones. Nevertheless, there are no available field studies regarding the impact of those conditions on fish muscle physiology. This work aimed to evaluate the muscle fibers size, protein content, gene expression of growth and atrophy-related genes in fish sampled from upwelling and downwelling zones. Seawater and fish food items (seaweeds) samples were collected from upwelling and downwelling zones to determine the habitat's physical-chemical variations and the abundance of biomolecules in seaweed tissue. In addition, white skeletal muscle samples were collected from an intertidal fish to analyze muscular histology, the growth pathways of protein kinase B and the extracellular signal-regulated kinase; and the gene expression of growth- (insulin-like growth factor 1 and myosin heavy-chain) and atrophy-related genes (F-box only protein 32 and muscle RING-finger protein-1). Upwelling zones revealed higher nutrients in seawater and higher protein content in seaweed than samples from downwelling zones. Moreover, fish from upwelling zones presented a greater size of muscle fibers and protein content compared to downwelling fish, associated with lower protein ubiquitination and gene expression of F-box only protein 32. Our data indicate an attenuated use of proteins as energy source in upwelling conditions favoring protein synthesis and muscle growth. This report shed lights of how oceanographic conditions may modulate food quality and fish muscle physiology in an integrated way, with high implications for marine conservation and sustainable fisheries management.
Spatio-temporal changes in the biochemical parameters of the fishery resource Concholepas concholepas (Gastropoda: Muricidae) in the Southeastern Pacific Ocean
The fishery resource Concholepas concholepas is a key species in the benthic marine ecosystems of the Southeastern Pacific Ocean. In the present study, the spatio-temporal changes in the biochemical parameters were evaluated for adult individuals of C. concholepas. They were sampled in summer and winter under upwelling and Non-upwelling conditions in two Management and Exploitation Areas for Benthic Resources of Valparaíso, Chile. The results indicated that the variation in the biochemical parameters is explained to a greater extent by the season. For example, in both upwelling and Nonupwelling conditions, we found a higher content of total lipids, proteins, glucose, and energy in winter than summer. Temporal variations can be explained by the reproductive process after summer season, in which the egg spawning require a greater energy reserves to be perform. Nonetheless, differences in the nutritional status of preys along seasons of the year, can also influence on these results. In fact, these variations in the biochemical parameters of C. concholepas may be indicative of an adaptive seasonal physiological response to the environment for maintaining an optimal energy budget year-round. Altogether, this knowledge will contribute to the Management and Exploitation Areas for Benthic Resources, improving regulatory measures during the annual period of captures and landings of C. concholepas, favoring a more sustainable fishery in the Southeastern Pacific Ocean within an ecosystem approach.
Seasonal changes in the biochemical composition of females and offspring of red squat lobster, Pleuroncodes monodon (Decapoda, Munididae), from the Southeastern Pacific
Pleuroncodes monodon is an important fishery resource and key species from the Southeastern Pacific. During its prolonged reproductive period (from February to December), ovigerous females are exposed to seasonal variation in environmental factors (e.g. temperature and food availability), with higher levels of these environmental parameters during summer and lower in winter, and must adjust their biochemistry to ensure their own survival and that of their embryos. The aim of this study was to analyse seasonal changes in the biochemical composition of ovigerous females and their eggs. The data show that the content of lipids, proteins and organic matter in the hepatopancreases of females was significantly higher in winter than in summer. Similarly, the lipid content of the eggs was significantly higher in winter than in summer, yet the protein content of the eggs had the opposite pattern with greater values found in summer. Consistent with the ‘reproductive energetic model’, the initial and final energy produced by the hepatopancreas showed significant seasonal variations, whereas female investment in egg production (in absolute values) did not show significant seasonal differences. The seasonal differences observed in the biochemistry, organic matter and energy content of the female hepatopancreases may be related to the storage of energy for growth and reproduction. Furthermore, the seasonal variation in the content of lipids, proteins and energy in eggs is likely related to embryogenesis and larval hatching in function with environmental conditions. Seasonal variation in the biochemical composition of P. monodon has direct effects on the marine food web and also upon fisheries.
Carry-over effects modulated by salinity during the early ontogeny of the euryhaline crab Hemigrapsus crenulatus from the Southeastern Pacific coast: Development time and carbon and energy content of offspring
Hemigrapsus crenulatus is a key species of coastal and estuarine ecosystems in the Southeastern Pacific and New Zealand. Since the gravid females-and their embryos-develop under conditions of variable salinity, we propose that low external salinity will be met with an increase in energy expenditures in order to maintain osmoregulation; subsequently, the use of energy reserves for reproduction will be affected. In this study, we investigate in H. crenulatus whether 1) the biomass and energy content of embryos is influenced by salinity experienced during oogenesis and embryogenesis and 2) how variation in the biomass and energy content of embryos affects larval energetic condition at hatching. Here at low salinity (5 PSU), egg-bearing females experienced massive and frequent egg losses, and therefore the development of their eggs during embryogenesis was not completed. In turn, at intermediate and high salinity (15 and 30 PSU) embryogenesis was completed, egg development was successful, and larvae were obtained. Consistently, larvae hatched from eggs produced and incubated at high salinity (30 PSU) were larger, had higher dry weight, and had increased carbon content and energy than larvae hatched from eggs produced at intermediate salinity (15 PSU). From these results, it is seen that the size and biomass of early life stages of H. crenulatus can be affected by environmental salinity experienced during oogenesis and embryogenesis, and this variation can then directly affect the energetic condition of offspring at birth. Therefore, this study reveals a “cascade effect” modulated by salinity during the early ontogeny.
Seasonal dynamics of biochemical composition and fatty acids of swordfish (Xiphias gladius) in the Southeast Pacific Ocean off the coast of Chile
In the Southeast Pacific Ocean, Xiphias gladius migrates through the Chilean coastal zone for feeding. Here, it forages for different prey items from autumn to spring, acquiring a great variety of energy and nutritional reserves. We evaluated seasonal variations in the biochemical reserves (i.e., contents of lipids, proteins, and glucose), total energy content and fatty acid profile of specimens captured during the austral autumn, winter, and spring. Our results show that higher amounts of lipids were found in the winter and spring, while protein and glucose were higher in the autumn. Thus, the energy content showed significant differences, with higher levels in winter and spring. Furthermore, the fatty acid profile was more diverse in the spring than the autumn and winter and was characterized by higher amounts of polyunsaturated fatty acids. These findings suggest that temporal changes in the biochemical reserves, total energy content and fatty acid profile support the idea of a “trophic migration” (i.e., the feeding period) established by the dynamics of fishery fleets. The high amounts of lipids and diverse fatty acid profile found in the spring could indicate the end of the trophic migration during this season. Thus, X. gladius may reach an optimum nutritional condition in the spring and make energetic adjustments to carry out its reproductive migration during the austral summer. Therefore, this species seems to meet the high energy demands of the reproductive season by foraging for a wide range of prey items from autumn to spring and storing an increased amount of lipids at the end of the feeding period. Overall, our data provides crucial baseline knowledge for future research on the ecophysiology of X. gladius, as well as for the management and conservation of this fishery resource under an ecosystem approach.
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