This study determined the optimal resistive forces for testing muscle capacities through the standard cycle ergometer test (1 resistive force applied) and a recently developed 2-point method (2 resistive forces used for force-velocity modelling). Twenty-six men were tested twice on maximal sprints performed on a leg cycle ergometer against 5 flywheel resistive forces (R1–R5). The reliability of the cadence and maximum power measured against the 5 individual resistive forces, as well as the reliability of the force-velocity relationship parameters obtained from the selected 2-point methods (R1–R2, R1–R3, R1–R4, and R1–R5), were compared. The reliability of outcomes obtained from individual resistive forces was high except for R5. As a consequence, the combination of R1 (≈175 rpm) and R4 (≈110 rpm) provided the most reliable 2-point method (CV: 1.46%–4.04%; ICC: 0.89–0.96). Although the reliability of power capacity was similar for the R1–R4 2-point method (CV: 3.18%; ICC: 0.96) and the standard test (CV: 3.31%; ICC: 0.95), the 2-point method should be recommended because it also reveals maximum force and velocity capacities. Finally, we conclude that the 2-point method in cycling should be based on 2 distant resistive forces, but avoiding cadences below 110 rpm.