This study sought to elucidate kinetic and kinematic differences between unloaded and band-assisted countermovement jumps (CMJs). In a randomized order, 20 healthy subjects (mass 84.5 ± 18.6 kg) completed 3 repetitions of CMJs across 3 conditions: unloaded (at body mass), low, and moderate band (8.4 ± 1.9 and 13.3 ± 3.3 kg body mass reduction, respectively). For all repetitions, a force platform and linear position transducer were used to record and calculate kinetic and kinematic data. Body mass was significantly different between the unloaded, low, and moderate band conditions (p < 0.05). Peak velocity, absolute peak, and mean force and movement duration displayed a trend that was mostly related to the condition (i.e., unloaded > low > moderate) (p < 0.05). The opposing trend (i.e., moderate > low > unloaded) was generally observed for relative peak and mean force, reactive strength index modified, and flight time (p < 0.05). No differences were observed for mean velocity, movement duration, and absolute and relative landing forces (p > 0.05). The use of band assistance during CMJs can alter force, time, and velocity variables. Practitioners should be aware of the potential positive and negative effects of band assistance during CMJs.