Aquifers are complex systems that present significant challenges in terms of characterization due to the lack or absence of watershed-scale hydrogeological information. An alternative to address the need to characterize watershed-scale aquifer behavior is recession flow analysis. Recession flows are flows sustained by groundwater release from the aquifer. Aquifer behavior can be characterized using recession flow records available from gauging stations, and therefore an indirect measure of aquifer behavior is obtained through watershed-scale recession flow records and analysis. This study seeks to identify the minimum time period necessary to characterize the behavior of groundwater storage systems in watersheds with different geological, morphological, and hydrological characteristics. To this end, various watersheds in south-central Chile underwent recession flow analysis, with eight time periods considered (2, 3, 4, 5, 10, 15, 20, and 25 years). The results indicate that 25 years of records are sufficient for the characterization of watershed-scale aquifer behavior, along with the representation of the groundwater storage-release (S-Q) process in watersheds with different geological, morphological, and hydrological characteristics. Additionally, the results show that an initial characterization of the groundwater system behavior in watersheds with different geological characteristics can be carried out with two years of records. This information could be important for practical engineering and the study of groundwater systems in watersheds with limited hydrological and hydrogeological information.