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  4. Effect of various dietary fructose concentrations on the gallstone formation process in mice
 
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Effect of various dietary fructose concentrations on the gallstone formation process in mice
Dra. Mardones-Leiva, Lorena 
Facultad de Medicina 
Dr. Villagrán-Orellana, Marcelo 
Facultad de Medicina 
Mg. Muñoz-Roa, Mirna 
Facultad de Medicina 
Del Pozo-Iribarren, Reginald
Facultad de Medicina 
Muñoz-Parra, Katia
Facultad de Medicina 
Troncoso-Ramírez, Luciano
Facultad de Medicina 
Mellado-Arias, Maximiliano
Facultad de Medicina 
10.20960/nh.04610
Arán Ediciones
2024
Background: little information is availaible on the effect of fructose on bile lipids. The first stage in the formation of gallstones corresponds to biliary cholesterol crystallization, derived from the vesicular transporters. The aim of this study was to investigate the influence of consuming diets with different fructose concentrations on serum lipids and their implications on gallstones formation.
Methods: BALB/c mice divided into a control group as well as groups were treated with different fructose concentrations (10 %, 30 %, 50 % or 70 %) for different periods (1, 2 or 5 months). Blood, liver and bile samples were obtained. In bile samples, cholesterol and phospholipids levels were analyzed, and cholesterol transporters (vesicles and micelles) were separated by gel filtration chromatography.
Results: treated animals showed: 1) increases in body weight similar to the control group; 2) a significant increase in plasma triglycerides only at very high fructose concentrations; 3) a significant increase in total serum cholesterol in the treatment for 1 month; 4) no variations in HDL-cholesterol; 5) a significant increase in serum glucose only at very high fructose concentrations in the second month of treatment; 6) no differences in the plasma alanine-aminotransferase activity; 7) a significant increase in liver triglyceride levels only at very high fructose concentrations; 8) no change in biliary lipid concentrations or in micellar and vesicular phospholipids.
Conclusion: changes in plasma, liver and bile lipids were only observed at very high fructose concentrations diets. We conclude that fructose apparently does not alter the gallstone formation process in our experimental model.
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Cholelithiasis
Dietary fructose
Lipid metabolism
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