Publication:
Skin-Mucus Prokaryote Community of Atlantic Salmon (Salmo salar) in Response to Bath Challenge With Tenacibaculum dicentrarchi

dc.contributor.authorAvendaño‐Herrera, Ruben
dc.contributor.authorTralma, Linette
dc.contributor.authorWicki, Hernán
dc.contributor.authorBarrios‐Henríquez, Fernanda
dc.contributor.authorLevipan, Héctor A.
dc.date.accessioned2025-07-04T01:01:20Z
dc.date.available2025-07-04T01:01:20Z
dc.date.issued2025
dc.description.abstractFish skin mucus is continuously replaced by epidermal cells, making it a highly dynamic microenvironment and an effective barrier against waterborne pathogens. The objective of this study was to understand the effects of tenacibaculosis, caused by the bacterium Tenacibaculum dicentrarchi, on the skin-associated microbiome of Atlantic salmon (Salmo salar). We used a vector-free and waterborne infection model of T. dicentrarchi strain TdCh05 in Atlantic salmon smolts for 21 days. Skin swab samples were collected at 2 h and 21 days post-infection (hpi and dpi, respectively) for 16S rRNA gene amplicon sequencing using DNA or complementary DNA (cDNA) as templates. Non-metric multidimensional scaling analysis grouped the samples into distinct clusters depending on the treatment and template. Similarity-Percentage (SIMPER) analysis indicated that between ~42% and 43% of the total amplicon sequence variants (ASVs) across all samples accounted for 90% of the compositional differences among all treatments and the two templates, highlighting the contribution of Tenacibaculum ASVs. Comparisons (by SIMPER) between non-infected and TdCh05-challenged fish at 2 hpi indicated that Tenacibaculum ASVs contributed to between ~52% and 58% of the differences in compositional clustering between samples. A significant drop in skin-mucus alpha diversity in TdCh05-challenged fish was also detected, followed by alpha diversity recovery at 21 dpi. In turn, at 21 dpi, microbiome changes were related to higher interaction complexity among taxa and community instability. Furthermore, 16S cDNA-based sequencing indicated that the potential activity of the Atlantic salmon skin-associated microbiome during disease progression was primarily driven by Tenacibaculum spp. Further research is needed to elucidate the role of other potentially active components (e.g., Pseudomonadales) of the skin-associated microbiome for the onset and/or progression of tenacibaculosis.
dc.identifier.doi10.1111/jfd.14157
dc.identifier.issn0140-7775
dc.identifier.issn1365-2761
dc.identifier.urihttps://repositorio.ucsc.cl/handle/25022009/12545
dc.languageeng
dc.publisherJohn Wiley & Sons Ltd
dc.relation.ispartofJournal of Fish Diseases
dc.relation.journalJournal of Fish Diseases
dc.rightsregistro bibliográfico
dc.subject16S cDNA-based amplicon sequencing
dc.subject16S DNA-based amplicon sequencing
dc.subjectAquaculture fish disease
dc.subjectFish skin dysbiosis
dc.subjectTenacibaculosis
dc.titleSkin-Mucus Prokaryote Community of Atlantic Salmon (Salmo salar) in Response to Bath Challenge With Tenacibaculum dicentrarchi
dc.typeartículo
dspace.entity.typePublication
oairecerif.author.affiliation#PLACEHOLDER_PARENT_METADATA_VALUE#
oairecerif.author.affiliationFacultad de Ciencias
oairecerif.author.affiliation#PLACEHOLDER_PARENT_METADATA_VALUE#
oairecerif.author.affiliation#PLACEHOLDER_PARENT_METADATA_VALUE#
oairecerif.author.affiliation#PLACEHOLDER_PARENT_METADATA_VALUE#
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